ISOLATION AND CHARACTERIZATION OF INFECTIOUS LARYNGOTRACHEITIS VIRUS IN LAYER CHICKENS

Isolation and Identification of Infectious Bronchitis Virus from Commercial Chickens

Laryngotracheitis Virus in Chickens

Infectious laryngotracheitis can be produced in chickens as an experimental model of severe nonfatal rhinitis and sinusitis. Inoculated intranasally into unanesthetized baby chicks it remains limited to the nasal fossa, produces acute desquamation of all nasal epithelia, results in functional recovery of the respiratory epithelium, but leaves important residual abnormalities. From the earliest ...

Histopathology of Infectious Laryngotracheitis in Chickens

1. The characteristic lesions of infectious laryngotracheitis are ordinarily restricted to the respiratory tract and are most pronounced in the larynx and trachea. Sometimes the eyelids are affected. A certain percentage of the cases are associated with bronchitis and peribronchitis, pneumonic areas and hemorrhages in the lung, while the involvement of the nasal passages, communicating sinuses,...

The role of mucosal antibody in immunity to infectious laryngotracheitis virus in chickens.

The role of mucosal antibody in recovery from a primary infection and resistance to reinfection with infectious laryngotracheitis (ILT) herpesvirus was studied in bursectomized chickens, which were unable to synthesize specific antibodies. Viral antigen in the infected trachea was assessed by indirect immunofluorescence on tissue sections and by ELISA. The ability of bursectomized chickens to r...

Detection of Infectious Laryngotracheitis Virus by Real-Time PCR in Naturally and Experimentally Infected Chickens

Infectious laryngotracheitis (ILT) is an acute, highly contagious upper-respiratory infectious disease of chickens. In this study, a real-time PCR method was developed for fast and accurate detection and quantitation of ILTV DNA of chickens experimentally infected with ILTV strain LJS09 and naturally infected chickens. The detection lower limit of the assay was 10 copies of DNA. There were no c...